Miltenyi Biotec Ultramicroscope II | Neuroscience Microscopy Service

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Image copyright Miltenyi Biotec 2021

For light-sheet imaging of cleared tissue volumes, we offer a Miltenyi / LaVision Biotec Ultramiscroscope with six bi-directional light sheets for optimal even coverage of large tissue volumes (e.g., Whole Brain, Whole Heart, etc.)

Fast-facts

  • Supports wide variety of aqueous [e.g. CLARITY] and organic [e.g., iDISCO+] solvents.
  • Refractive index matching 1.33–1.56 for optimal imaging
  • Large sample travel range (X, Y, Z): 1 cm x 1 cm x 1 cm
  • Lightsheet thickness 4–6 μm, width 1–20 mm (NA 0.0135–0.135)
  • Zoom microscope objective
    • 0.63× to 6.3× (1:10 zoom ratio)
    • 2× magnification, NA 0.5
    • FOV 1.7–17.6 mm (diagonal)
    • total magnification 1.7–17.6 (objective lens + zoom ratio) 
  • Fast image rate: 
  • Max. frame rate 100 fps @ 2560 × 2160 pixels (Full Frame: pixel size 6.5 × 6.5 μm)

Laser lines

405nm; 488nm; 561nm; 639nm; 785 nm (50–100 mW per diode)

Emission filters

Center wavelength/FWHM (nm): 460/40; 525/50; 620/60; 680/30; 845/55