LSM 710 Confocal Scope | Neuroscience Microscopy Service


The LSM 710 Confocal is an inverted laser-scanning confocal microscope. This scope is equipped with an environmental control chamber, fully automated motorized stage and software for time-lapse imaging of living cells.

Fast Facts

  • Fully automated microscope allowing tiled images, z-stacks and time-lapse imaging
  • Temperature, humidity, and CO2 control for live imaging.
  • Detection from 390 to 750 nm freely configurable
  • Brightfield, DIC and polarizer
  • Laser lines: 405nm; 488nm; 514nm; 568nm; 633 nm.


AxioObserver.Z1 Microscope

Z driveDC motorized Z-drive with optoelectronic coding, resolution 10 nm, reproducibility ±10 nm.
XY stageMotor-driven XY scanning stage (120 × 100 mm) with Mark & Find and Tile Scan (Mosaic Scan) functions; smallest increment 1 μm.
Transmitted light12 V/100 W halogen light source, motorized condenser LD 0.55 NA, brightfield/DIC/phase
Fluorescence equipmentEXFO X-Cite 120 illumination source, coupled to scope with liquid light guide, motorized 6-position reflector turret with DIC cube and three fluorescence filter cubes (see below).


10×Plan Apochromat0.452.0click here
20×Plan Apochromat0.80.55click here
40×Plan Neofluar (oil)1.30.21click here
63×Plan Apochromat (oil)1.40.19click here

Fluorescence filter cubes

Fluorescence filter cubes
(note: no camera, these are for eyepiece viewing only)
50% trans.
emissionpdf file
DAPI365/60 (peak/FWHM, nm)395420-470 (bandpass range, nm)DAPI_set49.pdf
GFP450-490 (bandpass range, nm)490500-550 (bandpass range, nm)GFP_set38HE.pdf
DsRed545/25 (peak/FWHM)570570-640 (bandpass range, nm)DsRed_set43.pdf

LSM 710 Scanning Module

ScannerTwo independent galvanometric scanning mirrors, real-time controlled.
Scan resolution4 × 1 to 6144 × 6144 pixels, continuously variable
Scanning speed14 × 2 speed stages; up to 12.5 frames/s with 256 × 256 pixels; 5 frames/s with 512 × 512 pixels; (max. 77 frames/s with 512 × 32 pixels), 0.38 ms for a line of 512 pixels; up to 2619 lines per second.
Scan zoom0.6× to 40×, digitally variable in steps of 0.1
Scan rotationFree 360° rotation in steps of 1°, free X/Y offset.
Scan field20 mm diagonal field (max.) in the intermediate image plane, with full pupil illumination
PinholesMaster pinhole pre-adjusted in size and position, individually variable for multi-tracking and short wavelengths (e.g. 405 nm)
DetectionThree simultaneous confocal fluorescence channels with highly sensitive low dark noise photomultiplier tubes (PMTs). Transmitted light channel with PMT.
Data depth8- 12- or 16-bit selectable

Laser Module

VIS Laser ModulePigtail-coupled lasers with polarization-preserving single-mode fibers, temperature-stabilized VIS-AOTF (acousto-optical tunable filter) for simultaneous intensity control; switching time <5 μs
Power [end-of-life specification]
Argon458, 488, 51425 or 35
DPSS (diode-pumped solid-state)56120

For a brief introduction to confocal_microscopy_from_Zeiss.pdf